Weiwei Bio for you to solve the problem of media preparation - Database & Sql Blog Articles

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In the middle of last month, several customers reached out to us with questions about media configuration.

At that time, our technical team provided them with detailed guidance on the configuration process and key precautions.

However, we realized that other customers might also face similar issues when preparing media.

Therefore, Chen Technology decided to share this information publicly, so that more users can benefit from our experience.

Here are some common problems and solutions related to media preparation:

Question 1: How to obtain a single colony by scribing? If it's not possible, what could be the reasons?

1. The plate may have too much moisture, which can interfere with the scribing process.

2. The inoculation loop wasn't properly sterilized or burned before use, leading to contamination or poor scribing.

3. Improper scribing technique, such as multi-zone scribing (three or four zones), may prevent the formation of distinct colonies.

Question 2: What is the difference between coating and pouring?

1. Coating allows for better observation of bacterial growth, but due to the small amount of bacteria applied, it may affect the accuracy of counting.

2. Pouring provides more accurate results, but it’s less ideal for observing colony morphology and behavior.

Question 3: What should you pay attention to when preparing the medium?

1. Sterilization temperature must be strictly controlled. For media with high sugar content, avoid excessively high temperatures, as this can cause caramelization and reduce quality.

2. Avoid repeated sterilization, as it can degrade nutrients and affect the overall performance of the medium.

3. After sterilization, make sure to mix the agar thoroughly to ensure even distribution.

This concludes today’s detailed explanation from Chen Technology. If you have any other questions during the media preparation process, feel free to contact us directly. Our technical team will be happy to assist you one-on-one. Thank you for your continued support and trust in our company. With your support, we will continue to provide higher quality products and services.