Weiwei Bio for you to solve the problem of media preparation - Database & Sql Blog Articles

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In the middle of last month, several customers reached out to us regarding media configuration issues.

At that time, our technical team provided detailed instructions and precautions for the configuration. However, considering that other customers may also face similar challenges, we decided to share this information publicly.

Our company, Chen Technology, is dedicated to helping each customer understand and resolve their specific issues during the medium preparation process.

Here are some common questions and solutions related to media preparation:

Question 1: How to obtain a single colony by scribing? What if it's not possible?

Reasons could include:

• There is too much moisture on the plate.
• The inoculation loop was not properly sterilized before marking.
• Multiple zones were used for scribing (e.g., three or four zones), which can lead to overlapping colonies.

Question 2: What’s the difference between coating and pouring?

Coating is better for observing the growth patterns of microorganisms, but because the amount of bacteria on the spreader is limited, it may affect the accuracy of counting. On the other hand, pouring allows for more accurate cell counting but is less ideal for observing colony morphology.

Question 3: What should you pay attention to when preparing the medium?

Important points include:

• Control the sterilization temperature strictly. For media with high sugar content, avoid excessively high temperatures, as this can cause caramelization and affect quality.
• Avoid repeated sterilization, as it may degrade nutrients over time.
• Make sure to mix the agar thoroughly after sterilization to ensure even distribution.

These are the key points from today’s detailed analysis by Chen Technology. If you have any other questions about media preparation, feel free to contact us directly. Our technical team will be happy to assist you one-on-one. Thank you for your continued support and trust in our company. With your support, we will keep improving and providing better products and services to you.